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Alternative name
Interleukin-12 subunit alpha ELISA KIT; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA KIT; CLMF p35 ELISA KIT; IL-12 subunit p35 ELISA KIT; NK cell stimulatory factor chain 1 ELISA KIT; NKSF1IL12A ELISA KIT; NKSF1 ELISA KIT; IL-12A ELISA KIT; CLMF p35 ELISA KIT; NKSF1 ELISA KIT
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Catalog
E020496
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species
Human
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GeneIL-12
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Other Species
Human IL-12 p35 ELISA KitHuman IL-12 p40 ELISA KitHuman IL-12 P70 ELISA KitHuman IL 12 ELISA KitHuman IL-12/P70 ELISA KitHuman IL-12/P40 ELISA KitMouse IL-12 p35 ELISA KitMouse IL-12 P70 ELISA KitMouse IL-12/P70 ELISA KitMouse IL-12 ELISA KitMouse IL-12/P40 ELISA Kit
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SpecificityNo cross-reaction with other factors.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity5pg/ml.
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Intended UseHuman IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human IL-12 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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