Human IRAK4 ELISA Kit

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  • Alternative name

    Human IRAK-4 ELISA Kit;Human renal carcinoma antigen NY-REN-64 ELISA Kit;Human IPD1 ELISA Kit;Human NY-REN-64 ELISA Kit;Human REN64 ELISA Kit;Human interleukin 1 receptor associated kinase 4 ELISA Kit;Human interleukin-1 receptor-associated kinase 4 ELISA Kit;

  • Catalog
    E020451
  • species
    Human
  • GeneIRAK4
  • Standard CurveHuman IRAK4 ELISA Kit
  • Other Species Mouse Irak4 ELISA KitBovine IRAK4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 4 (IRAK4). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 4 (IRAK4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.54ng/mL.
  • Intended UseHuman IRAK4 ELISA Kit allows for the in vitro quantitative determination of IRAK4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interleukin-1 receptor-associated kinase 4 Protein information
  • Uniprot ID IRAK4_HUMAN
  • Uniprot AC Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589;
  • UniGene Hs.138499;
  • GeneID 51135
  • KEGG hsa:51135;
  • Human Interleukin-1 receptor-associated kinase 4 Protein SEQUENCE
  • SEQUENCE 460 AA; 51530 MW; 6C8156ADF25FF81E CRC64;

    MNKPITPSTY VRCLNVGLIR KLSDFIDPQE GWKKLAVAIK KPSGDDRYNQ

    FHIRRFEALL QTGKSPTSEL LFDWGTTNCT VGDLVDLLIQ NEFFAPASLL

    LPDAVPKTAN TLPSKEAITV QQKQMPFCDK DRTLMTPVQN LEQSYMPPDS

    SSPENKSLEV SDTRFHSFSF YELKNVTNNF DERPISVGGN KMGEGGFGVV

    YKGYVNNTTV AVKKLAAMVD ITTEELKQQF DQEIKVMAKC QHENLVELLG

    FSSDGDDLCL VYVYMPNGSL LDRLSCLDGT PPLSWHMRCK IAQGAANGIN

    FLHENHHIHR DIKSANILLD EAFTAKISDF GLARASEKFA QTVMTSRIVG

    TTAYMAPEAL RGEITPKSDI YSFGVVLLEI ITGLPAVDEH REPQLLLDIK

    EEIEDEEKTI EDYIDKKMND ADSTSVEAMY SVASQCLHEK KNKRPDIKKV

    QQLLQEMTAS

  • UCSC uc001rnt.4; human. [Q9NWZ3-1];


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