Human IL-1ra/CD121 ELISA Kit

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  • Alternative name

    Interleukin-1 receptor type 1 ELISA KIT; CD121 antigen-like family member A ELISA KIT; Interleukin-1 receptor alpha ELISA KIT; IL-1R-alpha ELISA KIT; Interleukin-1 receptor type I ELISA KIT; p80IL1R1 ELISA KIT; IL1R ELISA KIT; IL1RA ELISA KIT; IL1RT1 ELISA KIT; IL-1R-1 ELISA KIT; IL-1RT-1 ELISA KIT; IL-1RT1 ELISA KIT; IL-1R-alpha ELISA KIT; mIL-1R1 ELISA KIT; mIL-1RI ELISA KIT; sIL-1R1 ELISA KIT; sIL-1RI ELISA KIT

  • Catalog
  • species
  • GeneIL-1ra/CD121
  • Standard CurveHuman IL-1ra/CD121 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman IL-1ra/CD121 ELISA Kit allows for the in vitro quantitative determination of IL-1ra/CD121 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    Intended Uses: This IL-1ra ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-1rain the sample, this IL-1raELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-1raconcentration. The concentration of IL-1rain the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This IL-1raenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-1ra. Standards or samples are then added to the microtiter plate wells and IL-1raif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-1rapresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-1raare added to each well to "sandwich" the IL-1raimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain IL-1raand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

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