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Alternative name
Interleukin-36 receptor antagonist protein ELISA KIT; FIL1 delta ELISA KIT; IL-1-related protein 3 ELISA KIT; IL-1RP3 ELISA KIT; Interleukin-1 HY1 ELISA KIT; IL-1HY1 ELISA KIT; Interleukin-1 delta ELISA KIT; IL-1 delta ELISA KIT; Interleukin-1 family member 5 ELISA KIT; IL-1F5 ELISA KIT; Interleukin-1 receptor antagonist homolog 1 ELISA KIT; IL-1ra homolog 1 ELISA KIT; Interleukin-1-like protein 1 ELISA KIT; IL-1L1IL36RN ELISA KIT; FIL1D ELISA KIT; IL1F5 ELISA KIT; IL1HY1 ELISA KIT; IL1L1 ELISA KIT; IL1RP3 ELISA KIT; UNQ1896/PRO4342 ELISA KIT; IL-1RP3 ELISA KIT; IL-1HY1 ELISA KIT; IL-1 delta ELISA KIT; IL-1F5 ELISA KIT; IL-1ra homolog 1 ELISA KIT; IL-1L1 ELISA KIT
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Catalog
E020423
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species
Human
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GeneIL1F5
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human IL1F5. No significant cross-reactivity or interference between human IL1F5 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human IL1F5 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseHuman IL1F5 ELISA Kit allows for the in vitro quantitative determination of IL1F5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1F5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1F5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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