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Human IRF1 ELISA Kit

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  • Alternative name

    Human IRF-1 ELISA Kit;Human MAR ELISA Kit;Human interferon regulatory factor 1 ELISA Kit;Human interferon regulatory factor 1 isoform +I9 ELISA Kit;Human interferon regulatory factor 1 isoform d78 ELISA Kit;Human interferon regulatory factor 1 isoform d9,10+ ELISA Kit;Human interferon regulatory factor 1 isoform delta4 ELISA Kit;Human interferon regulatory factor 1 isoform delta7 ELISA Kit;
  • Catalog
    E020323
  • species
    Human
  • GeneIRF1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Regulatory Factor 1 (IRF1). No significant cross-reactivity or interference between Interferon Regulatory Factor 1 (IRF1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman IRF1 ELISA Kit allows for the in vitro quantitative determination of IRF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 1 (IRF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 1 (IRF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 1 (IRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 1 (IRF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interferon regulatory factor 1 Protein information
  • Uniprot ID IRF1_HUMAN
  • Uniprot AC P10914; Q96GG7;
  • UniGene Hs.436061;
  • GeneID 3659
  • KEGG hsa:3659;
  • Human Interferon regulatory factor 1 Protein SEQUENCE

  • SEQUENCE 325 AA; 36502 MW; 2E06245A212D1541 CRC64;

    MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK

    DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN

    KGSSAVRVYR MLPPLTKNQR KERKSKSSRD AKSKAKRKSC GDSSPDTFSD

    GLSSSTLPDD HSSYTVPGYM QDLEVEQALT PALSPCAVSS TLPDWHIPVE

    VVPDSTSDLY NFQVSPMPST SEATTDEDEE GKLPEDIMKL LEQSEWQPTN

    VDGKGYLLNE PGVQPTSVYG DFSCKEEPEI DSPGGDIGLS LQRVFTDLKN

    MDATWLDSLL TPVRLPSIQA IPCAP




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