Human IFNE ELISA Kit

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  • Alternative name

    Human IFN-epsilon ELISA Kit;Human Interferon epsilon-1 ELISA Kit;Human IFNE1 ELISA Kit;Human IFN-E ELISA Kit;Human IFNT1 ELISA Kit;Human INFE1 ELISA Kit;Human PRO655 ELISA Kit;Human interferon epsilon ELISA Kit;Human interferon epsilon 1 ELISA Kit;Human interferon tau-1 ELISA Kit;

  • Catalog
    E020283
  • species
    Human
  • GeneIFNE
  • Standard CurveHuman IFNE ELISA Kit
  • Other Species Mouse Ifne ELISA KitPorcine IFNE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IFNepsilon. No significant cross-reactivity or interference between IFNepsilon and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNepsilon and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IFNE ELISA Kit allows for the in vitro quantitative determination of IFNE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Intended Uses: This IFNepsilon ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IFNepsilon. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||IFNepsilon ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNepsilon antibody and an IFNepsilon-HRP conjugate. The assay sample and buffer are incubated together with IFNepsilon-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNepsilon concentration since IFNepsilon from samples and IFNepsilon-HRP conjugate compete for the anti-IFNepsilon antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNepsilon from the sample, fewer sites are left to bind IFNepsilon-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNepsilon concentration in each sample is interpolated from this standard curve.
  • Human Interferon epsilon Protein information
  • Uniprot ID IFNE_HUMAN
  • Uniprot AC Q86WN2;
  • UniGene Hs.682604;
  • GeneID 338376
  • KEGG hsa:338376;
  • Human Interferon epsilon Protein SEQUENCE
  • SEQUENCE 208 AA; 24414 MW; 65FB861407F14E5F CRC64;

    MIIKHFFGTV LVLLASTTIF SLDLKLIIFQ QRQVNQESLK LLNKLQTLSI

    QQCLPHRKNF LLPQKSLSPQ QYQKGHTLAI LHEMLQQIFS LFRANISLDG

    WEENHTEKFL IQLHQQLEYL EALMGLEAEK LSGTLGSDNL RLQVKMYFRR

    IHDYLENQDY STCAWAIVQV EISRCLFFVF SLTEKLSKQG RPLNDMKQEL

    TTEFRSPR

  • UCSC uc003zpg.4; human.;


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