Human IFNA14 ELISA Kit

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  • Alternative name

    Human IFN-alpha-14 ELISA Kit;Human interferon alpha-H ELISA Kit;Human leIF H ELISA Kit;Human interferon lambda-2-H ELISA Kit;Human IFN-alphaH ELISA Kit;Human LEIF2H ELISA Kit;Human interferon alpha 14 ELISA Kit;Human interferon alpha-14 ELISA Kit;

  • Catalog
    E020257
  • species
    Human
  • GeneIFNA14
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4ng/ml
  • Intended UseHuman IFNA14 ELISA Kit allows for the in vitro quantitative determination of IFNA14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human IFNA14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IFNA14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFNA14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IFNA14 can be calculated.
  • Human Interferon alpha-14 Protein information
  • Uniprot ID IFN14_HUMAN
  • Uniprot AC VIQEVGVE ETPLMNEDSI LAVKKYFQRI TLYLMEKKYS PCAWEVVRAE IMRSLSFSTN
  • UniGene Hs.93907;
  • GeneID 3448
  • KEGG hsa:3448;
  • Human Interferon alpha-14 Protein SEQUENCE
  • SEQUENCE 189 AA; 22063 MW; B6B71E2F0D644FE7 CRC64;

    MALPFALMMA LVVLSCKSSC SLGCNLSQTH SLNNRRTLML MAQMRRISPF

    SCLKDRHDFE FPQEEFDGNQ FQKAQAISVL HEMMQQTFNL FSTKNSSAAW

    DETLLEKFYI ELFQQMNDLE ACVIQEVGVE ETPLMNEDSI LAVKKYFQRI

    TLYLMEKKYS PCAWEVVRAE IMRSLSFSTN LQKRLRRKD

  • UCSC uc010mis.4; human.;


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