Human IFNA21 ELISA Kit

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  • Alternative name

    Human IFN-alpha-21 ELISA Kit;Human interferon alpha-F ELISA Kit;Human LeIF F ELISA Kit;Human IFN-alphaI ELISA Kit;Human leIF-F ELISA Kit;Human interferon alpha 21 ELISA Kit;Human interferon alpha-21 ELISA Kit;Human leukocyte interferon protein ELISA Kit;

  • Catalog
    E020245
  • species
    Human
  • GeneIFNA21
  • Standard CurveHuman IFNA21 ELISA Kit
  • Other Species Mouse IFNA21 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IFNA21 ELISA Kit allows for the in vitro quantitative determination of IFNA21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: IFNA21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNA21 antibody and an IFNA21-HRP conjugate. The assay sample and buffer are incubated together with IFNA21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNA21 concentration since IFNA21 from samples and IFNA21-HRP conjugate compete for the anti-IFNA21 antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNA21 from the sample, fewer sites are left to bind IFNA21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNA21 concentration in each sample is interpolated from this standard curve.
  • Human Interferon alpha-21 Protein information
  • Uniprot ID IFN21_HUMAN
  • Uniprot AC VIQEVGVE ETPLMNVDSI LAVKKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSFSLSKI
  • UniGene Hs.113211;
  • GeneID 3452
  • KEGG hsa:3452;
  • Human Interferon alpha-21 Protein SEQUENCE
  • SEQUENCE 189 AA; 21741 MW; F0B6C9C392905802 CRC64;

    MALSFSLLMA VLVLSYKSIC SLGCDLPQTH SLGNRRALIL LAQMGRISPF

    SCLKDRHDFG FPQEEFDGNQ FQKAQAISVL HEMIQQTFNL FSTKDSSATW

    EQSLLEKFST ELNQQLNDLE ACVIQEVGVE ETPLMNVDSI LAVKKYFQRI

    TLYLTEKKYS PCAWEVVRAE IMRSFSLSKI FQERLRRKE

  • UCSC uc003zom.2; human.;


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