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Human IFN Alpha ELISA Kit

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  • Alternative name

    Interferon alpha-8 ELISA KIT; Interferon alpha-B ELISA KIT; LeIF B ELISA KIT; Interferon alpha-B2IFNA8 ELISA KIT; IFN-alpha-8 ELISA KIT; LeIF B ELISA KIT
  • Catalog
    E020229
  • species
    Human
  • GeneIFN Alpha
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IFN Alpha ELISA Kit allows for the in vitro quantitative determination of IFN Alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical    Principle of the Assay: alpha IFN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for alpha IFN. Standards or samples are then added to the microtiter plate wells and alpha IFN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alpha IFN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alpha IFN are added to each well to "sandwich" the alpha IFN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alpha IFN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha IFN concentration in each sample is interpolated from this standard curve.



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