Human ILK ELISA Kit

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  • Alternative name

    Human 59 kDa serine/threonine-protein kinase ELISA Kit;Human ILK-1 ELISA Kit;Human ILK-2 ELISA Kit;Human p59ILK ELISA Kit;Human ILK1 ELISA Kit;Human ILK2 ELISA Kit;Human HEL-S-28 ELISA Kit;Human P59 ELISA Kit;Human integrin linked kinase ELISA Kit;Human integrin-linked protein kinase ELISA Kit;Human epididymis secretory protein Li 28 ELISA Kit;Human integrin-linked kinase-2 ELISA Kit;

  • Catalog
    E020164
  • species
    Human
  • GeneILK
  • Other Species Human ILK 1 ELISA KitHuman ILK-1 ELISA KitMouse ILK-1 ELISA KitMouse Ilk ELISA KitBovine ILK ELISA KitGuinea Pig ILK ELISA KitRat Ilk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ILK. No significant cross-reactivity or interference between Human ILK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman ILK ELISA Kit allows for the in vitro quantitative determination of ILK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Integrin-linked kinase (ILK) is a 59kDa protein originally identified while conducting a yeast-two hybrid screen with integrin beta1 as the bait protein (Hannigan et al., 1996). Since its discovery, ILK has been associated with multiple cellular functions including cell migration, cell proliferation, cell-adhesions, signal transduction. ILK1 is a member of the serine/threonine kinases, has been demonstrated to be associated with numerous biological and pathological processes. However, the clinical and functional significance of ILK1 expression has not been characterized previously in human astrocytoma. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ILK-1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated ILK-1 and incubated. A competitive inhibition reaction is launched between ILK-1 (Standards or samples) and HRP-conjugated ILK-1 with the pre-coated antibody specific for ILK-1. The more amount of ILK-1 in samples, the less antibody bound by HRP-conjugated ILK-1. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ILK-1 in the sample. The color development is stopped and the intensity of the color is measured.
  • Human Integrin-linked protein kinase Protein information
  • Uniprot ID ILK_HUMAN
  • Uniprot AC Q13418; B7Z1I0; B7Z418; D3DQU0; P57043; Q68DZ3;
  • UniGene Hs.706355;
  • GeneID 3611
  • KEGG hsa:3611;
  • Human Integrin-linked protein kinase Protein SEQUENCE
  • SEQUENCE 452 AA; 51419 MW; E37DC2AD5311A1C2 CRC64;

    MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV

    EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG

    NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE

    RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLTKLN

    ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV

    LPVLGACQSP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD

    MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM

    YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI

    GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ

    DK

  • UCSC uc010rap.3; human. [Q13418-1];



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