Human ITG/CD11b ELISA Kit

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  • Alternative name

    FG-GAP repeat protein ELISA KIT; FG-GAP repeat protein

  • Catalog
  • species
  • GeneITG/CD11b
  • Standard CurveHuman ITG/CD11b ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman ITG/CD11b ELISA Kit allows for the in vitro quantitative determination of ITG/CD11b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This ITGalphaM /CD11b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ITGalphaM /CD11b. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ITGalphaM /CD11b ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITGalphaM /CD11b antibody and an ITGalphaM /CD11b-HRP onjugate. The assay sample and buffer are incubated together with ITGalphaM /CD11b-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITGalphaM /CD11b concentration since ITGalphaM /CD11b from samples and ITGalphaM /CD11b-HRP conjugate compete for the anti-ITGalphaM /CD11b antibody binding site. Since the number of sites is limited, as more sites are occupied by ITGalphaM /CD11b from the sample, fewer sites are left to bind ITGalphaM /CD11b-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITGalphaM /CD11b concentration in each sample is interpolated from this standard curve.

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