Human ITM2C ELISA Kit

Alternative name
Human cerebral protein 14 ELISA Kit;Human transmembrane protein BRI3 ELISA Kit;Human BRI3 ELISA Kit;Human BRICD2C ELISA Kit;Human E25 ELISA Kit;Human E25C ELISA Kit;Human ITM3 ELISA Kit;Human integral membrane protein 2C ELISA Kit;Human BRICHOS domain containing 2C ELISA Kit;Human integral membrane protein 3 ELISA Kit;
Catalog
E020057
species
Human
GeneITM2C
SpecificityThis assay has high sensitivity and excellent specificity for detection of ITM2C. No significant cross-reactivity or interference between ITM2C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITM2C and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ITM2C ELISA Kit allows for the in vitro quantitative determination of ITM2C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This ITM2C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ITM2C. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ITM2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ITM2C antibody and an ITM2C-HRP conjugate. The assay sample and buffer are incubated together with ITM2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2C concentration since ITM2C from samples and ITM2C-HRP conjugate compete for the anti-ITM2C antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2C from the sample, fewer sites are left to bind ITM2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2C concentration in each sample is interpolated from this standard curve.
Human CT-BRI3 Protein information
Uniprot ID ITM2C_HUMAN
Uniprot AC Q9BUP8;
UniGene Hs.111577;
GeneID 81618
KEGG hsa:81618;
Human CT-BRI3 Protein SEQUENCE
SEQUENCE 267 AA; 30224 MW; 234DDB91B4F282E7 CRC64;
MVKISFQPAV AGIKGDKADK ASASAPAPAS ATEILLTPAR EEQPPQHRSK
RGGSVGGVCY LSMGMVVLLM GLVFASVYIY RYFFLAQLAR DNFFRCGVLY
EDSLSSQVRT QMELEEDVKI YLDENYERIN VPVPQFGGGD PADIIHDFQR
GLTAYHDISL DKCYVIELNT TIVLPPRNFW ELLMNVKRGT YLPQTYIIQE
EMVVTEHVSD KEALGSFIYH LCNGKDTYRL RRRATRRRIN KRGAKNCNAI
RHFENTFVVE TLICGVV
UCSC uc002vqz.5; human. [Q9NQX7-1];

Human ITM2C ELISA Kit

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