Human ITM2B ELISA Kit

Alternative name
Human immature BRI2 ELISA Kit;Human imBRI2 ELISA Kit;Human Protein E25B ELISA Kit;Human transmembrane protein BRI ELISA Kit;Human BRI ELISA Kit;Human Mature BRI2 ELISA Kit;Human mBRI2 ELISA Kit;Human BRI2 ICD ELISA Kit;Human Bri2-23 ELISA Kit;Human ABri23 ELISA Kit;Human C-terminal peptide ELISA Kit;Human P23 peptide ELISA Kit;Human BRI2 ELISA Kit;Human ABRI ELISA Kit;Human BRICD2B ELISA Kit;Human E25B ELISA Kit;Human E3-16 ELISA Kit;Human FBD ELISA Kit;Human RDGCA ELISA Kit;Human integral membrane protein 2B ELISA Kit;Human ABri/ADan amyloid peptide ELISA Kit;Human BRICHOS domain containing 2B ELISA Kit;
Catalog
E020056
species
Human
GeneITM2B
SpecificityThis assay has high sensitivity and excellent specificity for detection of ITM2B. No significant cross-reactivity or interference between ITM2B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITM2B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ITM2B ELISA Kit allows for the in vitro quantitative determination of ITM2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: ITM2B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITM2B antibody and an ITM2B-HRP conjugate. The assay sample and buffer are incubated together with ITM2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2B concentration since ITM2B from samples and ITM2B-HRP conjugate compete for the anti-ITM2B antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2B from the sample, fewer sites are left to bind ITM2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2B concentration in each sample is interpolated from this standard curve.
Human Bri23 peptide Protein information
Uniprot ID ITM2B_HUMAN
Uniprot AC Q9Y287; Q5W0A3; Q96B24; Q9NYH1;
UniGene Hs.643683;
GeneID 9445
KEGG hsa:9445;
Human Bri23 peptide Protein SEQUENCE
SEQUENCE 266 AA; 30338 MW; 3A7D8CA259F1F627 CRC64;
MVKVTFNSAL AQKEAKKDEP KSGEEALIIP PDAVAVDCKD PDDVVPVGQR
RAWCWCMCFG LAFMLAGVIL GGAYLYKYFA LQPDDVYYCG IKYIKDDVIL
NEPSADAPAA LYQTIEENIK IFEEEEVEFI SVPVPEFADS DPANIVHDFN
KKLTAYLDLN LDKCYVIPLN TSIVMPPRNL LELLINIKAG TYLPQSYLIH
EHMVITDRIE NIDHLGFFIY RLCHDKETYK LQRRETIKGI QKREASNCFA
IRHFENKFAV ETLICS
UCSC uc001vbz.4; human. [Q9Y287-1];

Category

Human ITM2B ELISA Kit