Human IGFALS ELISA Kit

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  • Alternative name

    Human ALS ELISA Kit;Human ACLSD ELISA Kit;Human insulin like growth factor binding protein acid labile subunit ELISA Kit;Human insulin-like growth factor-binding protein complex acid labile subunit ELISA Kit;Human insulin-like growth factor binding protein complex acid labile chain ELISA Kit;

  • Catalog
    E019960
  • species
    Human
  • GeneIGFALS
  • Standard CurveHuman IGFALS ELISA Kit
  • Other Species Mouse Igfals ELISA KitRat Igfals ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.69ng/mL.
  • Intended UseHuman IGFALS ELISA Kit allows for the in vitro quantitative determination of IGFALS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Insulin-like growth factor-binding protein complex acid labile subunit Protein information
  • Uniprot ID ALS_HUMAN
  • Uniprot AC P35858; B4DZY8; E9PGU3;
  • UniGene Hs.839;
  • GeneID 3483
  • KEGG hsa:3483;
  • Human Insulin-like growth factor-binding protein complex acid labile subunit Protein SEQUENCE
  • SEQUENCE 605 AA; 66035 MW; F6562A23CBE918F6 CRC64;

    MALRKGGLAL ALLLLSWVAL GPRSLEGADP GTPGEAEGPA CPAACVCSYD

    DDADELSVFC SSRNLTRLPD GVPGGTQALW LDGNNLSSVP PAAFQNLSSL

    GFLNLQGGQL GSLEPQALLG LENLCHLHLE RNQLRSLALG TFAHTPALAS

    LGLSNNRLSR LEDGLFEGLG SLWDLNLGWN SLAVLPDAAF RGLGSLRELV

    LAGNRLAYLQ PALFSGLAEL RELDLSRNAL RAIKANVFVQ LPRLQKLYLD

    RNLIAAVAPG AFLGLKALRW LDLSHNRVAG LLEDTFPGLL GLRVLRLSHN

    AIASLRPRTF KDLHFLEELQ LGHNRIRQLA ERSFEGLGQL EVLTLDHNQL

    QEVKAGAFLG LTNVAVMNLS GNCLRNLPEQ VFRGLGKLHS LHLEGSCLGR

    IRPHTFTGLS GLRRLFLKDN GLVGIEEQSL WGLAELLELD LTSNQLTHLP

    HRLFQGLGKL EYLLLSRNRL AELPADALGP LQRAFWLDVS HNRLEALPNS

    LLAPLGRLRY LSLRNNSLRT FTPQPPGLER LWLEGNPWDC GCPLKALRDF

    ALQNPSAVPR FVQAICEGDD CQPPAYTYNN ITCASPPEVV GLDLRDLSEA

    HFAPC

  • UCSC uc002cmy.4; human. [P35858-1];


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