Human ILGF beta1 ELISA Kit

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  • Catalog
    E019935
  • species
    Human
  • GeneILGF beta1
  • Standard CurveHuman ILGF beta1 ELISA Kit
  • Other Species Mouse ILGF-beta1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ILGF beta1 ELISA Kit allows for the in vitro quantitative determination of ILGF beta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This IGF-B1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGF-B1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: IGF-B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF-B1 antibody and an IGF-B1 -HRP conjugate. The assay sample and buffer are incubated together with IGF-B1 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF-B1 concentration since IGF-B1 from samples and IGF-B1 -HRP conjugate compete for the anti-IGF-B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF-B1 from the sample, fewer sites are left to bind IGF-B1 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-B1 concentration in each sample is interpolated from this standard curve.


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