Human IGF1 ELISA Kit

Alternative name
Human IGF-I ELISA Kit;Human mechano growth factor ELISA Kit;Human MGF ELISA Kit;Human somatomedin-C ELISA Kit;Human IBP1 ELISA Kit;Human IGFI ELISA Kit;Human insulin like growth factor 1 ELISA Kit;Human insulin-like growth factor I ELISA Kit;Human insulin-like growth factor 1 (somatomedin C) ELISA Kit;Human insulin-like growth factor IB ELISA Kit;
Catalog
E019921
species
Human
GeneIGF1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.09ng/mL.
Intended UseHuman IGF1 ELISA Kit allows for the in vitro quantitative determination of IGF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Insulin-like growth factor I Protein information
Uniprot ID IGF1_HUMAN
Uniprot AC P05019; B2RWM7; E9PD02; P01343; Q14620;
UniGene Hs.160562;
GeneID 3479
KEGG hsa:3479;
Human Insulin-like growth factor I Protein SEQUENCE
SEQUENCE 195 AA; 21841 MW; E88A8CFBD1CD1873 CRC64;
MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP
ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC
DLRRLEMYCA PLKPAKSARS VRAQRHTDMP KTQKYQPPST NKNTKSQRRK
GWPKTHPGGE QKEGTEASLQ IRGKKKEQRR EIGSRNAECR GKKGK
UCSC uc001tjm.2; human. [P05019-1];

Human IGF1 ELISA Kit

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