Human IGF1 ELISA Kit

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  • Alternative name

    Human IGF-I ELISA Kit;Human mechano growth factor ELISA Kit;Human MGF ELISA Kit;Human somatomedin-C ELISA Kit;Human IBP1 ELISA Kit;Human IGFI ELISA Kit;Human insulin like growth factor 1 ELISA Kit;Human insulin-like growth factor I ELISA Kit;Human insulin-like growth factor 1 (somatomedin C) ELISA Kit;Human insulin-like growth factor IB ELISA Kit;

  • Catalog
    E019921
  • species
    Human
  • GeneIGF1
  • Standard CurveHuman IGF1 ELISA Kit
  • Other Species Mouse Igf1 ELISA KitBovine IGF1 ELISA KitCanine IGF1 ELISA KitGoat IGF1 ELISA KitGuinea Pig IGF1 ELISA KitChicken IGF1 ELISA KitHorse IGF1 ELISA KitPorcine IGF1 ELISA KitRabbit IGF1 ELISA KitRat Igf1 ELISA KitSheep IGF1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.09ng/mL.
  • Intended UseHuman IGF1 ELISA Kit allows for the in vitro quantitative determination of IGF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Insulin-like growth factor I Protein information
  • Uniprot ID IGF1_HUMAN
  • Uniprot AC P05019; B2RWM7; E9PD02; P01343; Q14620;
  • UniGene Hs.160562;
  • GeneID 3479
  • KEGG hsa:3479;
  • Human Insulin-like growth factor I Protein SEQUENCE
  • SEQUENCE 195 AA; 21841 MW; E88A8CFBD1CD1873 CRC64;

    MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP

    ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC

    DLRRLEMYCA PLKPAKSARS VRAQRHTDMP KTQKYQPPST NKNTKSQRRK

    GWPKTHPGGE QKEGTEASLQ IRGKKKEQRR EIGSRNAECR GKKGK

  • UCSC uc001tjm.2; human. [P05019-1];



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