Human IDE ELISA Kit

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  • Alternative name

    Human Abeta-degrading protease ELISA Kit;Human insulin protease ELISA Kit;Human insulinase ELISA Kit;Human INSULYSIN ELISA Kit;Human insulin degrading enzyme ELISA Kit;Human insulin-degrading enzyme ELISA Kit;

  • Catalog
    E019917
  • species
    Human
  • GeneIDE
  • Standard CurveHuman IDE ELISA Kit
  • Other Species Mouse Ide ELISA KitBovine IDE ELISA KitRat Ide ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IDE. No significant cross-reactivity or interference between IDE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.138ng/mL.
  • Intended UseHuman IDE ELISA Kit allows for the in vitro quantitative determination of IDE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IDE in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IDE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IDE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Insulin-degrading enzyme Protein information
  • Uniprot ID IDE_HUMAN
  • Uniprot AC P14735; B2R721; B7ZAU2; D3DR35; Q5T5N2;
  • UniGene Hs.500546;
  • GeneID 3416
  • KEGG hsa:3416;
  • Human Insulin-degrading enzyme Protein SEQUENCE
  • SEQUENCE 1019 AA; 117968 MW; 8A28AEF75EDA0EDA CRC64;

    MRYRLAWLLH PALPSTFRSV LGARLPPPER LCGFQKKTYS KMNNPAIKRI

    GNHITKSPED KREYRGLELA NGIKVLLISD PTTDKSSAAL DVHIGSLSDP

    PNIAGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY

    FDVSHEHLEG ALDRFAQFFL CPLFDESCKD REVNAVDSEH EKNVMNDAWR

    LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE LLKFHSAYYS

    SNLMAVCVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ

    LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL

    KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK

    LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGI LHYYPLEEVL

    TAEYLLEEFR PDLIEMVLDK LRPENVRVAI VSKSFEGKTD RTEEWYGTQY

    KQEAIPDEVI KKWQNADLNG KFKLPTKNEF IPTNFEILPL EKEATPYPAL

    IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL

    KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA

    TFEIDEKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL

    KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG IMQMVEDTLI

    EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ

    STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII

    QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL

    SAECAKYWGE IISQQYNFDR DNTEVAYLKT LTKEDIIKFY KEMLAVDAPR

    RHKVSVHVLA REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR

    GLPLFPLVKP HINFMAAKL

  • UCSC uc001kia.4; human. [P14735-1];


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