Human IMPDH2 ELISA Kit

Alternative name
Human IMP dehydrogenase 2 ELISA Kit;Human IMPD 2 ELISA Kit;Human IMPDH 2 ELISA Kit;Human IMPDH-II ELISA Kit;Human IMPD2 ELISA Kit;Human inosine monophosphate dehydrogenase 2 ELISA Kit;Human inosine-5'-monophosphate dehydrogenase 2 ELISA Kit;Human IMP (inosine 5'-monophosphate) dehydrogenase 2 ELISA Kit;Human IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit;Human IMP oxireductase 2 ELISA Kit;Human inosine 5' phosphate dehydrogenase 2 ELISA Kit;Human inosine monophosphate dehydrogenase type II ELISA Kit;
Catalog
E019667
species
Human
GeneIMPDH2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IMPDH2. No significant cross-reactivity or interference between Human IMPDH2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity5.8 pg/ml
Intended UseHuman IMPDH2 ELISA Kit allows for the in vitro quantitative determination of IMPDH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IMPDH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IMPDH2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IMPDH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IMPDH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Inosine-5'-monophosphate dehydrogenase 2 Protein information
Uniprot ID IMDH2_HUMAN
Uniprot AC P12268; Q6LEF3;
UniGene Hs.654400;
GeneID 3615
KEGG hsa:3615;
Human Inosine-5'-monophosphate dehydrogenase 2 Protein SEQUENCE
SEQUENCE 514 AA; 55805 MW; 876BEA0EC1DDBEE9 CRC64;
MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD
LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ
ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM
GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI
LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI
GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI
GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV
SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY
FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK
GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE
GGVHSLHSYE KRLF
UCSC uc003cvt.4; human.;

Category

Human IMPDH2 ELISA Kit