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Alternative name
Human Ig kappa chain V-IV region B17 ELISA Kit;Human Ig kappa chain V-IV region JI ELISA Kit;Human Ig kappa chain V-IV region Len ELISA Kit;Human Ig kappa chain V-IV region STH ELISA Kit;
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Catalog
E019625
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species
Human
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GeneIGKV4-1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human IGKV4-1. No significant cross-reactivity or interference between human IGKV4-1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human IGKV4-1 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
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Intended UseHuman IGKV4-1 ELISA Kit allows for the in vitro quantitative determination of IGKV4-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGKV4-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGKV4-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGKV4-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGKV4-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human Immunoglobulin kappa variable 4-1 Protein information
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Uniprot ID
KV401_HUMAN
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Uniprot AC
P06312; P01625; P06313; P06314; P83593;
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Human Immunoglobulin kappa variable 4-1 Protein SEQUENCE
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SEQUENCE 121 AA; 13380 MW; 9586AD4188D33974 CRC64;
MVLQTQVFIS LLLWISGAYG DIVMTQSPDS LAVSLGERAT INCKSSQSVL
YSSNNKNYLA
WYQQKPGQPP KLLIYWASTR ESGVPDRFSG SGSGTDFTLT
ISSLQAEDVA VYYCQQYYST
P
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UCSC
uc061lqi.1; human.;
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