Human IER3 ELISA Kit

Alternative name
Human differentiation-dependent gene 2 protein ELISA Kit;Human protein DIF-2 ELISA Kit;Human immediate early protein GLY96 ELISA Kit;Human immediate early response 3 protein ELISA Kit;Human PACAP-responsive gene 1 protein ELISA Kit;Human Protein PRG1 ELISA Kit;Human DIF2 ELISA Kit;Human IEX1 ELISA Kit;Human PRG1 ELISA Kit;Human DIF-2 ELISA Kit;Human GLY96 ELISA Kit;Human IEX-1 ELISA Kit;Human IEX-1L ELISA Kit;Human immediate early response 3 ELISA Kit;Human radiation-inducible immediate-early gene IEX-1 ELISA Kit;Human anti-death protein ELISA Kit;Human expressed in pancreatic carcinoma ELISA Kit;Human gly96, mouse, homolog of ELISA Kit;Human immediately early gene X-1 ELISA Kit;
Catalog
E019539
species
Human
GeneIER3
Standard Curve
Other Species Mouse Ier3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Immediate Early Response 3 (IER3). No significant cross-reactivity or interference between Immediate Early Response 3 (IER3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman IER3 ELISA Kit allows for the in vitro quantitative determination of IER3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immediate Early Response 3 (IER3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immediate Early Response 3 (IER3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immediate Early Response 3 (IER3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immediate Early Response 3 (IER3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Radiation-inducible immediate-early gene IEX-1 Protein information
Uniprot ID IEX1_HUMAN
Uniprot AC P46695; Q5SU30; Q92691; Q93044;
UniGene Hs.730861; Hs.76095;
GeneID 8870
KEGG hsa:8870;
Human Radiation-inducible immediate-early gene IEX-1 Protein SEQUENCE
SEQUENCE 156 AA; 16903 MW; 83C06116C81B8341 CRC64;
MCHSRSCHPT MTILQAPTPA PSTIPGPRRG SGPEIFTFDP LPEPAAAPAG
RPSASRGHRK RSRRVLYPRV VRRQLPVEEP NPAKRLLFLL LTIVFCQILM
AEEGVPAPLP PEDAPNAASL APTPVSAVLE PFNLTSEPSD YALDLSTFLQ
QHPAAF
UCSC uc003nrn.4; human.;

Category

Human IER3 ELISA Kit