Human IL7 ELISA Kit

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  • Alternative name

    Human IL-7 ELISA Kit;Human interleukin 7 ELISA Kit;Human interleukin-7 ELISA Kit;

  • Catalog
    E019513
  • species
    Human
  • GeneIL7
  • Standard CurveHuman IL7 ELISA Kit
  • Other Species Mouse Il7 ELISA KitBovine IL7 ELISA KitPorcine IL7 ELISA KitRat Il7 ELISA KitSheep IL7 ELISA Kit
  • SpecificityNatural and recombinant human IL-7
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman IL7 ELISA Kit allows for the in vitro quantitative determination of IL7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, D26-H177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-7 amount of sample captured in plate. Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that inhumans is encoded by the IL7 gene.Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.
  • Human Interleukin-7 Protein information
  • Uniprot ID IL7_HUMAN
  • Uniprot AC P13232; A0N0L3; Q5FBY5; Q5FBY9;
  • UniGene Hs.591873;
  • GeneID 3574
  • KEGG hsa:3574;
  • Human Interleukin-7 Protein SEQUENCE
  • SEQUENCE 177 AA; 20187 MW; 8FC5243F9169617F CRC64;

    MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD

    SMKEIGSNCL NNEFNFFKRH ICDANKEGMF LFRAARKLRQ FLKMNSTGDF

    DLHLLKVSEG TTILLNCTGQ VKGRKPAALG EAQPTKSLEE NKSLKEQKKL

    NDLCFLKRLL QEIKTCWNKI LMGTKEH

  • UCSC uc003ybg.3; human. [P13232-1];




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