Human IL5 ELISA Kit

Alternative name
Human IL-5 ELISA Kit;Human B-cell differentiation factor I ELISA Kit;Human eosinophil differentiation factor ELISA Kit;Human T-cell replacing factor ELISA Kit;Human TRF ELISA Kit;Human EDF ELISA Kit;Human interleukin 5 ELISA Kit;Human interleukin-5 ELISA Kit;Human colony-stimulating factor, eosinophil ELISA Kit;
Catalog
E019492
species
Human
GeneIL5
SpecificityThe Human IL-5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-5 proteins within the range of 3.9 pg/ml - 250 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity2 pg/ml.
Intended UseHuman IL5 ELISA Kit allows for the in vitro quantitative determination of IL5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
Human Interleukin-5 Protein information
Uniprot ID IL5_HUMAN
Uniprot AC P05113; Q13840;
UniGene Hs.2247;
GeneID 3567
KEGG hsa:3567;
Human Interleukin-5 Protein SEQUENCE
SEQUENCE 134 AA; 15238 MW; DC984467179556A3 CRC64;
MRMLLHLSLL ALGAAYVYAI PTEIPTSALV KETLALLSTH RTLLIANETL
RIPVPVHKNH QLCTEEIFQG IGTLESQTVQ GGTVERLFKN LSLIKKYIDG
QKKKCGEERR RVNQFLDYLQ EFLGVMNTEW IIES
UCSC uc003kxe.1; human.;

Human IL5 ELISA Kit

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