Human IL31 ELISA Kit

Alternative name
Human IL-31 ELISA Kit;Human interleukin 31 ELISA Kit;Human interleukin-31 ELISA Kit;
Catalog
E019468
species
Human
GeneIL31
SpecificityNatural and recombinant human IL-31
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL31 ELISA Kit allows for the in vitro quantitative determination of IL31 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, S24-T164) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-31 amount of sample captured in plate. Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.
Human Interleukin-31 Protein information
Uniprot ID IL31_HUMAN
Uniprot AC Q6EBC2; A2RUQ1;
UniGene Hs.569071;
GeneID 386653
KEGG hsa:386653;
Human Interleukin-31 Protein SEQUENCE
SEQUENCE 164 AA; 18205 MW; 1D61824CB6049445 CRC64;
MASHSGPSTS VLFLFCCLGG WLASHTLPVR LLRPSDDVQK IVEELQSLSK
MLLKDVEEEK GVLVSQNYTL PCLSPDAQPP NNIHSPAIRA YLKTIRQLDN
KSVIDEIIEH LDKLIFQDAP ETNISVPTDT HECKRFILTI SQQFSECMDL
ALKSLTSGAQ QATT
UCSC uc001ubv.3; human.;

Human IL31 ELISA Kit

Human IL-31 ELISA Kit;Human interleukin 31 ELISA Kit;Human interleukin-31 ELISA Kit;

Contact Us