Human IL3 ELISA Kit

Alternative name
Human IL-3 ELISA Kit;Human hematopoietic growth factor ELISA Kit;Human Mast cell growth factor ELISA Kit;Human MCGF ELISA Kit;Human multipotential colony-stimulating factor ELISA Kit;Human P-cell-stimulating factor ELISA Kit;Human MULTI-CSF ELISA Kit;Human interleukin 3 ELISA Kit;Human interleukin-3 ELISA Kit;Human P-cell stimulating factor ELISA Kit;Human colony-stimulating factor, multiple ELISA Kit;Human mast-cell growth factor ELISA Kit;Human multilineage-colony-stimulating factor ELISA Kit;
Catalog
E019463
species
Human
GeneIL3
SpecificityNatural and recombinant human IL-3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman IL3 ELISA Kit allows for the in vitro quantitative determination of IL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-3 amount of sample captured in plate. Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, ischemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors thatregulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 Themouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the longarm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 aminoacids with the molecular mass of 15KDa.
Human Interleukin-3 Protein information
Uniprot ID IL3_HUMAN
Uniprot AC P08700; Q6GS87;
UniGene Hs.694;
GeneID 3562
KEGG hsa:3562;
Human Interleukin-3 Protein SEQUENCE
SEQUENCE 152 AA; 17233 MW; BB46F23FAC1259A4 CRC64;
MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP
LPLLDFNNLN GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL
LPCLPLATAA PTRHPIHIKD GDWNEFRRKL TFYLKTLENA QAQQTTLSLA
IF
UCSC uc003kwe.2; human.;

Human IL3 ELISA Kit

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