Human ALK ELISA Kit

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  • Alternative name

    Human Anaplastic lymphoma kinase ELISA Kit;Human CD246 ELISA Kit;Human NBLST3 ELISA Kit;Human anaplastic lymphoma receptor tyrosine kinase ELISA Kit;Human ALK tyrosine kinase receptor ELISA Kit;Human CD246 antigen ELISA Kit;Human mutant anaplastic lymphoma kinase ELISA Kit;

  • Catalog
    E001946
  • species
    Human
  • GeneALK
  • Standard CurveHuman ALK ELISA Kit
  • Other Species Human Alk-Smase ELISA KitHuman alk Smase ELISA KitMouse Alk ELISA KitMouse Alk-Smase ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ALK. No significant cross-reactivity or interference between ALK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ALK ELISA Kit allows for the in vitro quantitative determination of ALK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical
    Intended Uses: This ALK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALK. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ALK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALK antibody and an ALK-HRP conjugate. The assay sample and buffer are incubated together with ALK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALK concentration since ALK from samples and ALK-HRP conjugate compete for the anti-ALK antibody binding site. Since the number of sites is limited, as more sites are occupied by ALK from the sample, fewer sites are left to bind ALK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALK concentration in each sample is interpolated from this standard curve.
  • Human ALK tyrosine kinase receptor Protein information
  • Uniprot ID ALK_HUMAN
  • Uniprot AC Q9UM73; Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6;
  • UniGene Hs.654469;
  • GeneID 238
  • KEGG hsa:238;
  • Human ALK tyrosine kinase receptor Protein SEQUENCE
  • SEQUENCE 1620 AA; 176442 MW; 0733D6C4FD212F41 CRC64;

    MGAIGLLWLL PLLLSTAAVG SGMGTGQRAG SPAAGPPLQP REPLSYSRLQ

    RKSLAVDFVV PSLFRVYARD LLLPPSSSEL KAGRPEARGS LALDCAPLLR

    LLGPAPGVSW TAGSPAPAEA RTLSRVLKGG SVRKLRRAKQ LVLELGEEAI

    LEGCVGPPGE AAVGLLQFNL SELFSWWIRQ GEGRLRIRLM PEKKASEVGR

    EGRLSAAIRA SQPRLLFQIF GTGHSSLESP TNMPSPSPDY FTWNLTWIMK

    DSFPFLSHRS RYGLECSFDF PCELEYSPPL HDLRNQSWSW RRIPSEEASQ

    MDLLDGPGAE RSKEMPRGSF LLLNTSADSK HTILSPWMRS SSEHCTLAVS

    VHRHLQPSGR YIAQLLPHNE AAREILLMPT PGKHGWTVLQ GRIGRPDNPF

    RVALEYISSG NRSLSAVDFF ALKNCSEGTS PGSKMALQSS FTCWNGTVLQ

    LGQACDFHQD CAQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTPQ

    WQVRTLKDAR FQDHQDHALL LSTTDVPASE SATVTSATFP APIKSSPCEL

    RMSWLIRGVL RGNVSLVLVE NKTGKEQGRM VWHVAAYEGL SLWQWMVLPL

    LDVSDRFWLQ MVAWWGQGSR AIVAFDNISI SLDCYLTISG EDKILQNTAP

    KSRNLFERNP NKELKPGENS PRQTPIFDPT VHWLFTTCGA SGPHGPTQAQ

    CNNAYQNSNL SVEVGSEGPL KGIQIWKVPA TDTYSISGYG AAGGKGGKNT

    MMRSHGVSVL GIFNLEKDDM LYILVGQQGE DACPSTNQLI QKVCIGENNV

    IEEEIRVNRS VHEWAGGGGG GGGATYVFKM KDGVPVPLII AAGGGGRAYG

    AKTDTFHPER LENNSSVLGL NGNSGAAGGG GGWNDNTSLL WAGKSLQEGA

    TGGHSCPQAM KKWGWETRGG FGGGGGGCSS GGGGGGYIGG NAASNNDPEM

    DGEDGVSFIS PLGILYTPAL KVMEGHGEVN IKHYLNCSHC EVDECHMDPE

    SHKVICFCDH GTVLAEDGVS CIVSPTPEPH LPLSLILSVV TSALVAALVL

    AFSGIMIVYR RKHQELQAMQ MELQSPEYKL SKLRTSTIMT DYNPNYCFAG

    KTSSISDLKE VPRKNITLIR GLGHGAFGEV YEGQVSGMPN DPSPLQVAVK

    TLPEVCSEQD ELDFLMEALI ISKFNHQNIV RCIGVSLQSL PRFILLELMA

    GGDLKSFLRE TRPRPSQPSS LAMLDLLHVA RDIACGCQYL EENHFIHRDI

    AARNCLLTCP GPGRVAKIGD FGMARDIYRA SYYRKGGCAM LPVKWMPPEA

    FMEGIFTSKT DTWSFGVLLW EIFSLGYMPY PSKSNQEVLE FVTSGGRMDP

    PKNCPGPVYR IMTQCWQHQP EDRPNFAIIL ERIEYCTQDP DVINTALPIE

    YGPLVEEEEK VPVRPKDPEG VPPLLVSQQA KREEERSPAA PPPLPTTSSG

    KAAKKPTAAE ISVRVPRGPA VEGGHVNMAF SQSNPPSELH KVHGSRNKPT

    SLWNPTYGSW FTEKPTKKNN PIAKKEPHDR GNLGLEGSCT VPPNVATGRL

    PGASLLLEPS SLTANMKEVP LFRLRHFPCG NVNYGYQQQG LPLEAATAPG

    AGHYEDTILK SKNSMNQPGP

  • UCSC uc002rmy.4; human.;




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