Human IL-2Ralpha/CD25 ELISA Kit

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  • Alternative name

    Interleukin-2 receptor subunit alpha ELISA KIT; TAC antigen ELISA KIT; p55 ELISA KIT; CD_antigen: CD25IL2RA ELISA KIT; IL-2 receptor subunit alpha ELISA KIT; IL-2-RA ELISA KIT; IL-2R subunit alpha ELISA KIT; IL2-RA ELISA KIT

  • Catalog
  • species
  • GeneIL-2Ralpha/CD25
  • SpecificityThis kit recognizes natural and recombinant Human IL-2Ralpha/CD25. No significant cross-reactivity or interference between Human IL-2Ralpha/CD25 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human IL-2Ralpha/CD25 is 56.25pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman IL-2Ralpha/CD25 ELISA Kit allows for the in vitro quantitative determination of IL-2Ralpha/CD25 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-2Ralpha/CD25. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-2Ralpha/CD25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-2Ralpha/CD25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-2Ralpha/CD25. You can calculate the concentration of IL-2Ralpha/CD25 in the samples by comparing the OD of the samples to the standard curve.

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