Human IL22 ELISA Kit

Alternative name
Human IL-22 ELISA Kit;Human cytokine Zcyto18 ELISA Kit;Human IL-10-related T-cell-derived-inducible factor ELISA Kit;Human IL-TIF ELISA Kit;Human ILTIF ELISA Kit;Human zcyto18 ELISA Kit;Human IL-21 ELISA Kit;Human IL-D110 ELISA Kit;Human TIFIL-23 ELISA Kit;Human TIFa ELISA Kit;Human interleukin 22 ELISA Kit;Human interleukin-22 ELISA Kit;Human IL-10-related T-cell-derived inducible factor ELISA Kit;
Catalog
E019434
species
Human
GeneIL22
SpecificityNatural and recombinant human IL-22
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL22 ELISA Kit allows for the in vitro quantitative determination of IL22 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards(E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-22 amount of sample captured in plate. Background: Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Human Interleukin-22 Protein information
Uniprot ID IL22_HUMAN
Uniprot AC Q9GZX6;
UniGene Hs.287369;
GeneID 50616
KEGG hsa:50616;
Human Interleukin-22 Protein SEQUENCE
SEQUENCE 179 AA; 20011 MW; 3C35E64D60CF8767 CRC64;
MAALQKSVSS FLMGTLATSC LLLLALLVQG GAAAPISSHC RLDKSNFQQP
YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL
EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD
TVKKLGESGE IKAIGELDLL FMSLRNACI
UCSC uc001sty.2; human.;

Human IL22 ELISA Kit

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