Human IL20 ELISA Kit

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  • Alternative name

    Human IL-20 ELISA Kit;Human cytokine Zcyto10 ELISA Kit;Human ZCYTO10 ELISA Kit;Human IL10D ELISA Kit;Human interleukin 20 ELISA Kit;Human interleukin-20 ELISA Kit;Human four alpha helix cytokine ELISA Kit;

  • Catalog
    E019429
  • species
    Human
  • GeneIL20
  • Other Species Mouse Il20 ELISA Kit
  • SpecificityNatural and recombinant human IL-20
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IL20 ELISA Kit allows for the in vitro quantitative determination of IL20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli,L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-20 amount of sample captured in plate. Background: Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
  • Human Interleukin-20 Protein information
  • Uniprot ID IL20_HUMAN
  • Uniprot AC Q9NYY1; Q17RB3; Q2THG6; Q96QZ6;
  • UniGene Hs.272373;
  • GeneID 50604
  • KEGG hsa:50604;
  • Human Interleukin-20 Protein SEQUENCE
  • SEQUENCE 176 AA; 20072 MW; 8385992500B6C447 CRC64;

    MKASSLAFSL LSAAFYLLWT PSTGLKTLNL GSCVIATNLQ EIRNGFSEIR

    GSVQAKDGNI DIRILRRTES LQDTKPANRC CLLRHLLRLY LDRVFKNYQT

    PDHYTLRKIS SLANSFLTIK KDLRLCHAHM TCHCGEEAMK KYSQILSHFE

    KLEPQAAVVK ALGELDILLQ WMEETE

  • UCSC uc001her.4; human. [Q9NYY1-1];



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