Human IL2 ELISA Kit

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  • Alternative name

    Human IL-2 ELISA Kit;Human T-cell growth factor ELISA Kit;Human TCGF ELISA Kit;Human lymphokine ELISA Kit;Human interleukin 2 ELISA Kit;Human interleukin-2 ELISA Kit;Human T cell growth factor ELISA Kit;Human aldesleukin ELISA Kit;Human involved in regulation of T-cell clonal expansion ELISA Kit;

  • Catalog
    E019418
  • species
    Human
  • GeneIL2
  • Standard CurveHuman IL2 ELISA Kit
  • Other Species Human IL2-Ab ELISA KitMouse Il2 ELISA KitBovine IL2 ELISA KitCanine IL2 ELISA KitGoat IL2 ELISA KitHorse IL2 ELISA KitMonkey IL2 ELISA KitPorcine IL2 ELISA KitRabbit IL2 ELISA KitRat Il2 ELISA KitSheep IL2 ELISA Kit
  • SpecificityNatural and recombinant human IL-2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman IL2 ELISA Kit allows for the in vitro quantitative determination of IL2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-2 amount of sample captured in plate. Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for bothB and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Human IL-2 is a15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
  • Human Interleukin-2 Protein information
  • Uniprot ID IL2_HUMAN
  • Uniprot AC P60568; P01585;
  • UniGene Hs.89679;
  • GeneID 3558
  • KEGG hsa:3558;
  • Human Interleukin-2 Protein SEQUENCE
  • SEQUENCE 153 AA; 17628 MW; 59E2F40F25860F84 CRC64;

    MYRMQLLSCI ALSLALVTNS APTSSSTKKT QLQLEHLLLD LQMILNGINN

    YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL

    RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS

    TLT




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