Human IL1RN ELISA Kit

Alternative name
Human IL-1RN ELISA Kit;Human IL-1ra ELISA Kit;Human IRAP ELISA Kit;Human ICIL-1RA ELISA Kit;Human IL1 inhibitor ELISA Kit;Human IL1F3 ELISA Kit;Human IL1RA ELISA Kit;Human DIRA ELISA Kit;Human IL-1ra3 ELISA Kit;Human MVCD4 ELISA Kit;Human interleukin 1 receptor antagonist ELISA Kit;Human interleukin-1 receptor antagonist protein ELISA Kit;Human intracellular IL-1 receptor antagonist type II ELISA Kit;Human intracellular interleukin-1 receptor antagonist (icIL-1ra) ELISA Kit;Human type II interleukin-1 receptor antagonist ELISA Kit;
Catalog
E019414
species
Human
GeneIL1RN
SpecificityNatural and recombinant human IL-1RA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman IL1RN ELISA Kit allows for the in vitro quantitative determination of IL1RN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli,R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate. Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene.1.2Thehuman IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities ofinterleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.4
Human Interleukin-1 receptor antagonist protein Protein information
Uniprot ID IL1RA_HUMAN
Uniprot AC P18510; A8K4G1; Q14628; Q53SC2; Q7RTZ4; Q96GD6; Q9UPC0;
UniGene Hs.81134;
GeneID 3557
KEGG hsa:3557;
Human Interleukin-1 receptor antagonist protein Protein SEQUENCE
SEQUENCE 177 AA; 20055 MW; D1690776A7394057 CRC64;
MEICRGLRSH LITLLLFLFH SETICRPSGR KSSKMQAFRI WDVNQKTFYL
RNNQLVAGYL QGPNVNLEEK IDVVPIEPHA LFLGIHGGKM CLSCVKSGDE
TRLQLEAVNI TDLSENRKQD KRFAFIRSDS GPTTSFESAA CPGWFLCTAM
EADQPVSLTN MPDEGVMVTK FYFQEDE
UCSC uc002tiy.3; human. [P18510-1];

Human IL1RN ELISA Kit

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