Human IL25 ELISA Kit

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  • Alternative name

    Human IL-25 ELISA Kit;Human interleukin-17E ELISA Kit;Human IL-17E ELISA Kit;Human IL17E ELISA Kit;Human interleukin 25 ELISA Kit;Human interleukin-25 ELISA Kit;

  • Catalog
    E019382
  • species
    Human
  • GeneIL25
  • Other Species Mouse IL25 ELISA Kit
  • SpecificityThe Human IL-17E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-17E proteins within the range of 62.5 pg/ml - 4000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman IL25 ELISA Kit allows for the in vitro quantitative determination of IL25 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human IL-17E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17E in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-17E coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-kappaB activation, and stimulate the production of IL8. What's more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
  • Human Interleukin-25 Protein information
  • Uniprot ID IL25_HUMAN
  • Uniprot AC Q9H293; Q2M3F0; Q8IZV3; Q8WXB0;
  • UniGene Hs.302036;
  • GeneID 64806
  • KEGG hsa:64806;
  • Human Interleukin-25 Protein SEQUENCE
  • SEQUENCE 177 AA; 20330 MW; 52D895710CD59871 CRC64;

    MRERPRLGED SSLISLFLQV VAFLAMVMGT HTYSHWPSCC PSKGQDTSEE

    LLRWSTVPVP PLEPARPNRH PESCRASEDG PLNSRAISPW RYELDRDLNR

    LPQDLYHARC LCPHCVSLQT GSHMDPRGNS ELLYHNQTVF YRRPCHGEKG

    THKGYCLERR LYRVSLACVC VRPRVMG

  • UCSC uc001wjq.4; human. [Q9H293-1];



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