Human IL17A ELISA Kit

Alternative name
Human IL-17 ELISA Kit;Human IL-17A ELISA Kit;Human cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit;Human CTLA-8 ELISA Kit;Human CTLA8 ELISA Kit;Human IL17 ELISA Kit;Human interleukin 17A ELISA Kit;Human interleukin-17A ELISA Kit;Human cytotoxic T-lymphocyte-associated protein 8 ELISA Kit;Human interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) ELISA Kit;
Catalog
E019372
species
Human
GeneIL17A
SpecificityNatural and recombinant human IL-17
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman IL17A ELISA Kit allows for the in vitro quantitative determination of IL17A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17 has been precoated onto 96-well plates. Standards (E.coli,I20-A155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17 amount of sample captured in plate. Background: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.
Human Interleukin-17A Protein information
Uniprot ID IL17_HUMAN
Uniprot AC Q16552; Q5T2P0;
UniGene Hs.41724;
GeneID 3605
KEGG hsa:3605;
Human Interleukin-17A Protein SEQUENCE
SEQUENCE 155 AA; 17504 MW; 2BCAE9CB2F4886D1 CRC64;
MTPGKTSLVS LLLLLSLEAI VKAGITIPRN PGCPNSEDKN FPRTVMVNLN
IHNRNTNTNP KRSSDYYNRS TSPWNLHRNE DPERYPSVIW EAKCRHLGCI
NADGNVDYHM NSVPIQQEIL VLRREPPHCP NSFRLEKILV SVGCTCVTPI
VHHVA
UCSC uc003pak.1; human.;

Human IL17A ELISA Kit

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