Human IL15 ELISA Kit

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  • Alternative name

    Human IL-15 ELISA Kit;Human interleukin 15 ELISA Kit;Human interleukin-15 ELISA Kit;

  • Catalog
    E019364
  • species
    Human
  • GeneIL15
  • Standard CurveHuman IL15 ELISA Kit
  • Other Species Mouse Il15 ELISA KitBovine IL15 ELISA KitGuinea Pig IL15 ELISA KitPorcine IL15 ELISA KitRabbit IL15 ELISA KitRat Il15 ELISA KitSheep IL15 ELISA Kit
  • SpecificityNatural and recombinant human IL-15
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 3pg/ml
  • Intended UseHuman IL15 ELISA Kit allows for the in vitro quantitative determination of IL15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IL-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-15 has been precoated onto 96-well plates. Standards(E.coli,N49-S162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-15 amount of sample captured in plate. Background: Interleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor.1 IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection.2 The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.
  • Human Interleukin-15 Protein information
  • Uniprot ID IL15_HUMAN
  • Uniprot AC P40933; D3DNZ2; O00440; O43512; Q495Z8; Q6FGX7; Q93058; Q9UBA3;
  • UniGene Hs.168132; Hs.602618; Hs.735901;
  • GeneID 3600
  • KEGG hsa:3600;
  • Human Interleukin-15 Protein SEQUENCE
  • SEQUENCE 162 AA; 18086 MW; 0CE0520C1D8379E2 CRC64;

    MRISKPHLRS ISIQCYLCLL LNSHFLTEAG IHVFILGCFS AGLPKTEANW

    VNVISDLKKI EDLIQSMHID ATLYTESDVH PSCKVTAMKC FLLELQVISL

    ESGDASIHDT VENLIILANN SLSSNGNVTE SGCKECEELE EKNIKEFLQS

    FVHIVQMFIN TS

  • UCSC uc003iis.4; human. [P40933-1];



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