Human IL13 ELISA Kit

Alternative name
Human IL-13 ELISA Kit;Human NC30 ELISA Kit;Human P600 ELISA Kit;Human interleukin 13 ELISA Kit;Human interleukin-13 ELISA Kit;
Catalog
E019359
species
Human
GeneIL13
SpecificityThe Human IL-13 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-13 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseHuman IL13 ELISA Kit allows for the in vitro quantitative determination of IL13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.
Human Interleukin-13 Protein information
Uniprot ID IL13_HUMAN
Uniprot AC P35225; O43644; Q4VB52; Q9UDC7;
UniGene Hs.845;
GeneID 3596
KEGG hsa:3596;
Human Interleukin-13 Protein SEQUENCE
SEQUENCE 146 AA; 15816 MW; BE2D46CCF913D129 CRC64;
MHPLLNPLLL ALGLMALLLT TVIALTCLGG FASPGPVPPS TALRELIEEL
VNITQNQKAP LCNGSMVWSI NLTAGMYCAA LESLINVSGC SAIEKTQRML
SGFCPHKVSA GQFSSLHVRD TKIEVAQFVK DLLLHLKKLF REGRFN
UCSC uc003kxj.2; human.;

Human IL13 ELISA Kit

Human IL-13 ELISA Kit;Human NC30 ELISA Kit;Human P600 ELISA Kit;Human interleukin 13 ELISA Kit;Human interleukin-13 ELISA Kit;

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