Human IL12A ELISA Kit

Alternative name
Human IL-12A ELISA Kit;Human cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit;Human CLMF p35 ELISA Kit;Human IL-12 subunit p35 ELISA Kit;Human NK cell stimulatory factor chain 1 ELISA Kit;Human NKSF1 ELISA Kit;Human CLMF ELISA Kit;Human NFSK ELISA Kit;Human P35 ELISA Kit;Human interleukin 12A ELISA Kit;Human interleukin-12 subunit alpha ELISA Kit;Human IL-12, subunit p35 ELISA Kit;Human IL35 subunit ELISA Kit;Human NF cell stimulatory factor chain 1 ELISA Kit;Human cytotoxic lymphocyte maturation factor 1, p35 ELISA Kit;Human interleukin 12, p35 ELISA Kit;Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ELISA Kit;Human interleukin-12 alpha chain ELISA Kit;
Catalog
E019348
species
Human
GeneIL12A
Other Species Mouse Il12a ELISA Kit Bovine IL12A ELISA Kit Canine IL12A ELISA Kit Goat IL12A ELISA Kit Horse IL12A ELISA Kit Porcine IL12A ELISA Kit Rat Il12a ELISA Kit Sheep IL12A ELISA Kit
SpecificityThe Human IL-12 p70 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-12 p70 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7 pg/ml.
Intended UseHuman IL12A ELISA Kit allows for the in vitro quantitative determination of IL12A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-12 p70 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin (IL)-12 is a 70 kDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12 p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.
Human Interleukin-12 subunit alpha Protein information
Uniprot ID IL12A_HUMAN
Uniprot AC P29459; Q96QZ1;
UniGene Hs.673;
GeneID 3592
KEGG hsa:3592;
Human Interleukin-12 subunit alpha Protein SEQUENCE
SEQUENCE 219 AA; 24874 MW; 7C658AB7716112B2 CRC64;
MCPARSLLLV ATLVLLDHLS LARNLPVATP DPGMFPCLHH SQNLLRAVSN
MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR
ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK
RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH
AFRIRAVTID RVMSYLNAS
UCSC uc003fcx.4; human.;

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