Human IL10 ELISA Kit

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  • Alternative name

    Human IL-10 ELISA Kit;Human cytokine synthesis inhibitory factor ELISA Kit;Human CSIF ELISA Kit;Human GVHDS ELISA Kit;Human IL10A ELISA Kit;Human TGIF ELISA Kit;Human interleukin 10 ELISA Kit;Human interleukin-10 ELISA Kit;Human T-cell growth inhibitory factor ELISA Kit;

  • Catalog
    E019331
  • species
    Human
  • GeneIL10
  • Other Species Mouse Il10 ELISA KitBovine IL10 ELISA KitCanine IL10 ELISA KitGuinea Pig IL10 ELISA KitChicken IL10 ELISA KitHorse IL10 ELISA KitMonkey IL10 ELISA KitPorcine IL10 ELISA KitRabbit IL10 ELISA KitRat Il10 ELISA KitSheep IL10 ELISA Kit
  • SpecificityThe Human IL-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-10 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity7 pg/ml.
  • Intended UseHuman IL10 ELISA Kit allows for the in vitro quantitative determination of IL10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
  • Human Interleukin-10 Protein information
  • Uniprot ID IL10_HUMAN
  • Uniprot AC P22301;
  • UniGene Hs.193717;
  • GeneID 3586
  • KEGG hsa:3586;
  • Human Interleukin-10 Protein SEQUENCE
  • SEQUENCE 178 AA; 20517 MW; 6825E9FA4337CDE4 CRC64;

    MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR

    VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN

    QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ

    EKGIYKAMSE FDIFINYIEA YMTMKIRN




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