Human IL10 ELISA Kit

Alternative name
Human IL-10 ELISA Kit;Human cytokine synthesis inhibitory factor ELISA Kit;Human CSIF ELISA Kit;Human GVHDS ELISA Kit;Human IL10A ELISA Kit;Human TGIF ELISA Kit;Human interleukin 10 ELISA Kit;Human interleukin-10 ELISA Kit;Human T-cell growth inhibitory factor ELISA Kit;
Catalog
E019331
species
Human
GeneIL10
SpecificityThe Human IL-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-10 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7 pg/ml.
Intended UseHuman IL10 ELISA Kit allows for the in vitro quantitative determination of IL10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
Human Interleukin-10 Protein information
Uniprot ID IL10_HUMAN
Uniprot AC P22301;
UniGene Hs.193717;
GeneID 3586
KEGG hsa:3586;
Human Interleukin-10 Protein SEQUENCE
SEQUENCE 178 AA; 20517 MW; 6825E9FA4337CDE4 CRC64;
MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR
VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN
QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ
EKGIYKAMSE FDIFINYIEA YMTMKIRN

Human IL10 ELISA Kit

Human IL-10 ELISA Kit;Human cytokine synthesis inhibitory factor ELISA Kit;Human CSIF ELISA Kit;Human GVHDS ELISA Kit;Human IL10A ELISA Kit;Human TGIF ELISA Kit;Human interleukin 10 ELISA Kit;Human interleukin-10 ELISA Kit;Human T-cell growth inhibitory factor ELISA Kit;

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