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Human IL1A ELISA Kit

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  • Alternative name

    Human IL-1 alpha ELISA Kit;Human hematopoietin-1 ELISA Kit;Human IL1F1 ELISA Kit;Human IL-1A ELISA Kit;Human IL1 ELISA Kit;Human IL1-ALPHA ELISA Kit;Human interleukin 1 alpha ELISA Kit;Human interleukin-1 alpha ELISA Kit;Human preinterleukin 1 alpha ELISA Kit;Human pro-interleukin-1-alpha ELISA Kit;
  • Catalog
    E019316
  • species
    Human
  • GeneIL1A
  • Standard CurveHuman IL1A ELISA Kit
  • Other Species Mouse Il1a ELISA KitBovine IL1A ELISA KitCanine IL1A ELISA KitGoat IL1A ELISA KitGuinea Pig IL1A ELISA KitHorse IL1A ELISA KitMonkey IL1A ELISA KitPorcine IL1A ELISA KitRabbit IL1A ELISA KitRat Il1a ELISA KitSheep IL1A ELISA Kit
  • SpecificityNatural and recombinant human IL-1alpha
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.5pg/ml
  • Intended UseHuman IL1A ELISA Kit allows for the in vitro quantitative determination of IL1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IL-1alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1alpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1alpha amount of sample captured in plate. Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha hasbeen implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has beenshown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene isassigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recentlysuggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encodestructurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1alpha with the molecular massof 18kDa.
  • Human Interleukin-1 alpha Protein information
  • Uniprot ID IL1A_HUMAN
  • Uniprot AC P01583; Q53QF9; Q7RU02;
  • UniGene Hs.1722;
  • GeneID 3552
  • KEGG hsa:3552;
  • Human Interleukin-1 alpha Protein SEQUENCE

  • SEQUENCE 271 AA; 30607 MW; CBCCC49569D9ED40 CRC64;

    MAKVPDMFED LKNCYSENEE DSSSIDHLSL NQKSFYHVSY GPLHEGCMDQ

    SVSLSISETS KTSKLTFKES MVVVATNGKV LKKRRLSLSQ SITDDDLEAI

    ANDSEEEIIK PRSAPFSFLS NVKYNFMRII KYEFILNDAL NQSIIRANDQ

    YLTAAALHNL DEAVKFDMGA YKSSKDDAKI TVILRISKTQ LYVTAQDEDQ

    PVLLKEMPEI PKTITGSETN LLFFWETHGT KNYFTSVAHP NLFIATKQDY

    WVCLAGGPPS ITDFQILENQ A

  • UCSC uc002tig.4; human.;


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