SpecificityThis assay has high sensitivity and excellent specificity for detection of IGHG1. No significant cross-reactivity or interference between IGHG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGHG1 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman IGHGI ELISA Kit allows for the in vitro quantitative determination of IGHGI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: IGHG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing IGHG1 antigen and an IGHG1-Ab-HRP conjugate. The assay sample and buffer are incubated together with IGHG1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGHG1 concentration since IGHG1 from samples and IGHG1 pre-coated on the plate compete for the IGHG1 Ab-HRP binding site.Since the number of sites is limited, as more sites are occupied by IGHG1 from the sample, fewer sites are left to bind IGHG1 pre-coated on the plate.A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGHG1 concentration in each sample is interpolated from this standard curve.