Human IGFBP6 ELISA Kit

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  • Alternative name

    Human IBP-6 ELISA Kit;Human IGF-binding protein 6 ELISA Kit;Human IGFBP-6 ELISA Kit;Human IBP6 ELISA Kit;Human insulin like growth factor binding protein 6 ELISA Kit;Human insulin-like growth factor-binding protein 6 ELISA Kit;Human IGF binding protein 6 ELISA Kit;

  • Catalog
    E019296
  • species
    Human
  • GeneIGFBP6
  • Other Species Mouse Igfbp6 ELISA KitBovine IGFBP6 ELISA KitRat Igfbp6 ELISA Kit
  • SpecificityNatural and recombinant human IGFBP6
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IGFBP6 ELISA Kit allows for the in vitro quantitative determination of IGFBP6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NSO, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP6 amount of sample captured in plate. Background: Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
  • Human Insulin-like growth factor-binding protein 6 Protein information
  • Uniprot ID IBP6_HUMAN
  • Uniprot AC P24592; Q14492;
  • UniGene Hs.274313;
  • GeneID 3489
  • KEGG hsa:3489;
  • Human Insulin-like growth factor-binding protein 6 Protein SEQUENCE
  • SEQUENCE 240 AA; 25322 MW; 285308231C025009 CRC64;

    MTPHRLLPPL LLLLALLLAA SPGGALARCP GCGQGVQAGC PGGCVEEEDG

    GSPAEGCAEA EGCLRREGQE CGVYTPNCAP GLQCHPPKDD EAPLRALLLG

    RGRCLPARAP AVAEENPKES KPQAGTARPQ DVNRRDQQRN PGTSTTPSQP

    NSAGVQDTEM GPCRRHLDSV LQQLQTEVYR GAQTLYVPNC DHRGFYRKRQ

    CRSSQGQRRG PCWCVDRMGK SLPGSPDGNG SSSCPTGSSG

  • UCSC uc001sbu.2; human.;




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