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Human IGFBP2 ELISA Kit

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  • Alternative name

    Human IBP-2 ELISA Kit;Human IGF-binding protein 2 ELISA Kit;Human IGFBP-2 ELISA Kit;Human BP2 ELISA Kit;Human IBP2 ELISA Kit;Human IGF-BP53 ELISA Kit;Human insulin like growth factor binding protein 2 ELISA Kit;Human insulin-like growth factor-binding protein 2 ELISA Kit;Human insulin-like growth factor binding protein 2, 36kDa ELISA Kit;
  • Catalog
    E019291
  • species
    Human
  • GeneIGFBP2
  • SpecificityThe Human IGFBP2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IGFBP2 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human IGFBP2 is typically less than 10 pg/ml.
  • Intended UseHuman IGFBP2 ELISA Kit allows for the in vitro quantitative determination of IGFBP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The Bioscience Human IGFBP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways is regulators of cancer-mediated endothelial recruitment.
  • Human Insulin-like growth factor-binding protein 2 Protein information
  • Uniprot ID IBP2_HUMAN
  • Uniprot AC P18065; Q14619; Q9UCL3;
  • UniGene Hs.438102;
  • GeneID 3485
  • KEGG hsa:3485;
  • Human Insulin-like growth factor-binding protein 2 Protein SEQUENCE

  • SEQUENCE 325 AA; 34814 MW; 9E1436DBCCC6EA2A CRC64;

    MLPRVGCPAL PLPPPPLLPL LLLLLGASGG GGGARAEVLF RCPPCTPERL

    AACGPPPVAP PAAVAAVAGG ARMPCAELVR EPGCGCCSVC ARLEGEACGV

    YTPRCGQGLR CYPHPGSELP LQALVMGEGT CEKRRDAEYG ASPEQVADNG

    DDHSEGGLVE NHVDSTMNML GGGGSAGRKP LKSGMKELAV FREKVTEQHR

    QMGKGGKHHL GLEEPKKLRP PPARTPCQQE LDQVLERIST MRLPDERGPL

    EHLYSLHIPN CDKHGLYNLK QCKMSLNGQR GECWCVNPNT GKLIQGAPTI

    RGDPECHLFY NEQQEARGVH TQRMQ

  • UCSC uc061sgd.1; human.;



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