Human IGFBP1 ELISA Kit

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  • Alternative name

    Human IBP-1 ELISA Kit;Human IGF-binding protein 1 ELISA Kit;Human IGFBP-1 ELISA Kit;Human placental protein 12 ELISA Kit;Human PP12 ELISA Kit;Human IBP1 ELISA Kit;Human AFBP ELISA Kit;Human IGF-BP25 ELISA Kit;Human hIGFBP-1 ELISA Kit;Human insulin like growth factor binding protein 1 ELISA Kit;Human insulin-like growth factor-binding protein 1 ELISA Kit;Human alpha-pregnancy-associated endometrial globulin ELISA Kit;Human amniotic fluid binding protein ELISA Kit;Human binding protein-25 ELISA Kit;Human binding protein-26 ELISA Kit;Human binding protein-28 ELISA Kit;Human growth hormone independent-binding protein ELISA Kit;

  • Catalog
    E019280
  • species
    Human
  • GeneIGFBP1
  • Standard CurveHuman IGFBP1 ELISA Kit
  • Other Species Mouse Igfbp1 ELISA KitBovine IGFBP1 ELISA KitPorcine IGFBP1 ELISA KitRat Igfbp1 ELISA Kit
  • SpecificityNatural and recombinant human IGFBP-1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman IGFBP1 ELISA Kit allows for the in vitro quantitative determination of IGFBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO,A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate. Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesizedby human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copygene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consistingof 234 amino acids with the molecular mass of 25KDa..
  • Human Insulin-like growth factor-binding protein 1 Protein information
  • Uniprot ID IBP1_HUMAN
  • Uniprot AC P08833; A4D2F4; D3DVL9; Q8IYP5;
  • UniGene Hs.642938;
  • GeneID 3484
  • KEGG hsa:3484;
  • Human Insulin-like growth factor-binding protein 1 Protein SEQUENCE
  • SEQUENCE 259 AA; 27904 MW; 8AA75AF7DC966012 CRC64;

    MSEVPVARVW LVLLLLTVQV GVTAGAPWQC APCSAEKLAL CPPVSASCSE

    VTRSAGCGCC PMCALPLGAA CGVATARCAR GLSCRALPGE QQPLHALTRG

    QGACVQESDA SAPHAAEAGS PESPESTEIT EEELLDNFHL MAPSEEDHSI

    LWDAISTYDG SKALHVTNIK KWKEPCRIEL YRVVESLAKA QETSGEEISK

    FYLPNCNKNG FYHSRQCETS MDGEAGLCWC VYPWNGKRIP GSPEIRGDPN

    CQIYFNVQN

  • UCSC uc003tnp.4; human.;



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