Human IgA1 ELISA Kit

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  • Alternative name

    Immunoglobulin A1 protease autotransporter ELISA KIT; Helper peptideiga ELISA KIT; iga1 ELISA KIT; IGA1 protease ELISA KIT

  • Catalog
    E019258
  • species
    Human
  • GeneIgA1
  • Other Species Mouse IGA1 ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human IgA1. No significant cross-reactivity or interference between Human IgA1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human IgA1 is 28.125ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman IgA1 ELISA Kit allows for the in vitro quantitative determination of IgA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IgA1. You can calculate the concentration of IgA1 in the samples by comparing the OD of the samples to the standard curve.



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