Intended UseHuman IGIP ELISA Kit allows for the in vitro quantitative determination of IGIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IgA-inducing protein homolog, During the reaction, IgA-inducing protein homolog in the sample or standard competes with a fixed amount of biotin-labeled IgA-inducing protein homolog for sites on a pre-coated Monoclonal antibody specific to IgA-inducing protein homolog. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IgA-inducing protein homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human IgA-inducing protein homolog Protein information
Human IgA-inducing protein homolog Protein SEQUENCE