SpecificityThe Human IFN alpha 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IFN alpha 1 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Intended UseHuman IFNA1 ELISA Kit allows for the in vitro quantitative determination of IFNA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||The Human IFN alpha 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IFN alpha 1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IFN alpha 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN alpha 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IFN alpha 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN alpha 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection; they also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: alpha, beta and gamma. IFN-alpha is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. When binding to discrete cell surface receptors on target cells, IFN-alpha induces rapid changes in Jak/Stat phosphorylation, which intiates the Jak/Stat signaling pathway. IFN-alpha signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon). All IFN-alpha signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.