Human IDUA ELISA Kit

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  • Alternative name

    Human IDA ELISA Kit;Human MPS1 ELISA Kit;Human iduronidase, alpha-L- ELISA Kit;Human alpha-L-iduronidase ELISA Kit;

  • Catalog
    E019234
  • species
    Human
  • GeneIDUA
  • Other Species Mouse Idua ELISA KitCanine IDUA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IDUA. No significant cross-reactivity or interference between Human IDUA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 ng/ml
  • Intended UseHuman IDUA ELISA Kit allows for the in vitro quantitative determination of IDUA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDUA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IDUA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IDUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IDUA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alpha-L-iduronidase Protein information
  • Uniprot ID IDUA_HUMAN
  • Uniprot AC P35475; B3KWK6;
  • UniGene Hs.89560;
  • GeneID 3425
  • KEGG hsa:3425;
  • Human Alpha-L-iduronidase Protein SEQUENCE
  • SEQUENCE 653 AA; 72670 MW; 9D2399B22FD172BD CRC64;

    MRPLRPRAAL LALLASLLAA PPVAPAEAPH LVHVDAARAL WPLRRFWRST

    GFCPPLPHSQ ADQYVLSWDQ QLNLAYVGAV PHRGIKQVRT HWLLELVTTR

    GSTGRGLSYN FTHLDGYLDL LRENQLLPGF ELMGSASGHF TDFEDKQQVF

    EWKDLVSSLA RRYIGRYGLA HVSKWNFETW NEPDHHDFDN VSMTMQGFLN

    YYDACSEGLR AASPALRLGG PGDSFHTPPR SPLSWGLLRH CHDGTNFFTG

    EAGVRLDYIS LHRKGARSSI SILEQEKVVA QQIRQLFPKF ADTPIYNDEA

    DPLVGWSLPQ PWRADVTYAA MVVKVIAQHQ NLLLANTTSA FPYALLSNDN

    AFLSYHPHPF AQRTLTARFQ VNNTRPPHVQ LLRKPVLTAM GLLALLDEEQ

    LWAEVSQAGT VLDSNHTVGV LASAHRPQGP ADAWRAAVLI YASDDTRAHP

    NRSVAVTLRL RGVPPGPGLV YVTRYLDNGL CSPDGEWRRL GRPVFPTAEQ

    FRRMRAAEDP VAAAPRPLPA GGRLTLRPAL RLPSLLLVHV CARPEKPPGQ

    VTRLRALPLT QGQLVLVWSD EHVGSKCLWT YEIQFSQDGK AYTPVSRKPS

    TFNLFVFSPD TGAVSGSYRV RALDYWARPG PFSDPVPYLE VPVPRGPPSP

    GNP

  • UCSC uc003gby.5; human. [P35475-1];


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