Human APLP1 ELISA Kit

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  • Alternative name

    Human APLP ELISA Kit;Human APLP-1 ELISA Kit;Human amyloid beta precursor like protein 1 ELISA Kit;Human amyloid-like protein 1 ELISA Kit;Human amyloid beta (A4) precursor-like protein 1 ELISA Kit;Human amyloid precursor-like protein 1 ELISA Kit;

  • Catalog
    E001923
  • species
    Human
  • GeneAPLP1
  • Standard CurveHuman APLP1 ELISA Kit
  • Other Species Mouse Aplp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of APLP1. No significant cross-reactivity or interference between APLP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APLP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman APLP1 ELISA Kit allows for the in vitro quantitative determination of APLP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This APLP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human APLP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||APLP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APLP1 antibody and an APLP1-HRP conjugate. The assay sample and buffer are incubated together with APLP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APLP1 concentration since APLP1 from samples and APLP1-HRP conjugate compete for the anti-APLP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APLP1 from the sample, fewer sites are left to bind APLP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APLP1 concentration in each sample is interpolated from this standard curve.
  • Human C30 Protein information
  • Uniprot ID APLP1_HUMAN
  • Uniprot AC P51693; O00113; Q96A92;
  • UniGene Hs.74565;
  • GeneID 333
  • KEGG hsa:333;
  • Human C30 Protein SEQUENCE
  • SEQUENCE 650 AA; 72176 MW; B95F0F4D1C5CBAC7 CRC64;

    MGPASPAARG LSRRPGQPPL PLLLPLLLLL LRAQPAIGSL AGGSPGAAEA

    PGSAQVAGLC GRLTLHRDLR TGRWEPDPQR SRRCLRDPQR VLEYCRQMYP

    ELQIARVEQA TQAIPMERWC GGSRSGSCAH PHHQVVPFRC LPGEFVSEAL

    LVPEGCRFLH QERMDQCESS TRRHQEAQEA CSSQGLILHG SGMLLPCGSD

    RFRGVEYVCC PPPGTPDPSG TAVGDPSTRS WPPGSRVEGA EDEEEEESFP

    QPVDDYFVEP PQAEEEEETV PPPSSHTLAV VGKVTPTPRP TDGVDIYFGM

    PGEISEHEGF LRAKMDLEER RMRQINEVMR EWAMADNQSK NLPKADRQAL

    NEHFQSILQT LEEQVSGERQ RLVETHATRV IALINDQRRA ALEGFLAALQ

    ADPPQAERVL LALRRYLRAE QKEQRHTLRH YQHVAAVDPE KAQQMRFQVH

    THLQVIEERV NQSLGLLDQN PHLAQELRPQ IQELLHSEHL GPSELEAPAP

    GGSSEDKGGL QPPDSKDDTP MTLPKGSTEQ DAASPEKEKM NPLEQYERKV

    NASVPRGFPF HSSEIQRDEL APAGTGVSRE AVSGLLIMGA GGGSLIVLSM

    LLLRRKKPYG AISHGVVEVD PMLTLEEQQL RELQRHGYEN PTYRFLEERP

  • UCSC uc002ocf.4; human. [P51693-1];


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