Human IDS ELISA Kit

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  • Alternative name

    Human alpha-L-iduronate sulfate sulfatase ELISA Kit;Human idursulfase ELISA Kit;Human SIDS ELISA Kit;Human MPS2 ELISA Kit;Human iduronate 2-sulfatase ELISA Kit;Human iduronate 2-sulfatase 14 kDa chain ELISA Kit;Human iduronate 2-sulfatase 42 kDa chain ELISA Kit;

  • Catalog
    E019225
  • species
    Human
  • GeneIDS
  • Standard CurveHuman IDS ELISA Kit
  • Other Species Mouse Ids ELISA Kit
  • SpecificityNatural and recombinant human IDS
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human IDS in cell culture supernates, serum and plasma
  • Intended UseHuman IDS ELISA Kit allows for the in vitro quantitative determination of IDS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate. Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
  • Human Iduronate 2-sulfatase 14 kDa chain Protein information
  • Uniprot ID IDS_HUMAN
  • Uniprot AC P22304; D3DWT4; Q14604; Q9BRM3;
  • UniGene Hs.460960; Hs.6795;
  • GeneID 3423
  • KEGG hsa:3423;
  • Human Iduronate 2-sulfatase 14 kDa chain Protein SEQUENCE
  • SEQUENCE 550 AA; 61873 MW; EA1B713417280413 CRC64;

    MPPPRTGRGL LWLGLVLSSV CVALGSETQA NSTTDALNVL LIIVDDLRPS

    LGCYGDKLVR SPNIDQLASH SLLFQNAFAQ QAVCAPSRVS FLTGRRPDTT

    RLYDFNSYWR VHAGNFSTIP QYFKENGYVT MSVGKVFHPG ISSNHTDDSP

    YSWSFPPYHP SSEKYENTKT CRGPDGELHA NLLCPVDVLD VPEGTLPDKQ

    STEQAIQLLE KMKTSASPFF LAVGYHKPHI PFRYPKEFQK LYPLENITLA

    PDPEVPDGLP PVAYNPWMDI RQREDVQALN ISVPYGPIPV DFQRKIRQSY

    FASVSYLDTQ VGRLLSALDD LQLANSTIIA FTSDHGWALG EHGEWAKYSN

    FDVATHVPLI FYVPGRTASL PEAGEKLFPY LDPFDSASQL MEPGRQSMDL

    VELVSLFPTL AGLAGLQVPP RCPVPSFHVE LCREGKNLLK HFRFRDLEED

    PYLPGNPREL IAYSQYPRPS DIPQWNSDKP SLKDIKIMGY SIRTIDYRYT

    VWVGFNPDEF LANFSDIHAG ELYFVDSDPL QDHNMYNDSQ GGDLFQLLMP

  • UCSC uc011mxe.3; human. [P22304-1];


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