Human ICAM3 ELISA Kit

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  • Alternative name

    Human ICAM-3 ELISA Kit;Human CDW50 ELISA Kit;Human ICAM-R ELISA Kit;Human CD50 ELISA Kit;Human intercellular adhesion molecule 3 ELISA Kit;

  • Catalog
    E019216
  • species
    Human
  • GeneICAM3
  • Other Species Bovine ICAM3 ELISA Kit
  • SpecificityNatural and recombinant human ICAM-3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman ICAM3 ELISA Kit allows for the in vitro quantitative determination of ICAM3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human ICAM-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-3 has been precoated onto 96-well plates. Standards(NSO, Q30-H485, homodimer) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-3 amount of sample captured in plate. Background: Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.
  • Human Intercellular adhesion molecule 3 Protein information
  • Uniprot ID ICAM3_HUMAN
  • Uniprot AC P32942; Q6PD68;
  • UniGene Hs.654563;
  • GeneID 3385
  • KEGG hsa:3385;
  • Human Intercellular adhesion molecule 3 Protein SEQUENCE
  • SEQUENCE 547 AA; 59541 MW; 4B7BDC02F24F3031 CRC64;

    MATMVPSVLW PRACWTLLVC CLLTPGVQGQ EFLLRVEPQN PVLSAGGSLF

    VNCSTDCPSS EKIALETSLS KELVASGMGW AAFNLSNVTG NSRILCSVYC

    NGSQITGSSN ITVYRLPERV ELAPLPPWQP VGQNFTLRCQ VEDGSPRTSL

    TVVLLRWEEE LSRQPAVEEP AEVTATVLAS RDDHGAPFSC RTELDMQPQG

    LGLFVNTSAP RQLRTFVLPV TPPRLVAPRF LEVETSWPVD CTLDGLFPAS

    EAQVYLALGD QMLNATVMNH GDTLTATATA TARADQEGAR EIVCNVTLGG

    ERREARENLT VFSFLGPIVN LSEPTAHEGS TVTVSCMAGA RVQVTLDGVP

    AAAPGQPAQL QLNATESDDG RSFFCSATLE VDGEFLHRNS SVQLRVLYGP

    KIDRATCPQH LKWKDKTRHV LQCQARGNPY PELRCLKEGS SREVPVGIPF

    FVNVTHNGTY QCQASSSRGK YTLVVVMDIE AGSSHFVPVF VAVLLTLGVV

    TIVLALMYVF REHQRSGSYH VREESTYLPL TSMQPTEAMG EEPSRAE

  • UCSC uc002mob.3; human.;



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