Human ICAM2 ELISA Kit

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  • Alternative name

    Human ICAM-2 ELISA Kit;Human CD102 ELISA Kit;Human intercellular adhesion molecule 2 ELISA Kit;

  • Catalog
    E019215
  • species
    Human
  • GeneICAM2
  • Other Species Mouse Icam2 ELISA Kit
  • SpecificityNatural and recombinant human ICAM-2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman ICAM2 ELISA Kit allows for the in vitro quantitative determination of ICAM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human ICAM-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-2 has been precoated onto 96-well plates. Standards(NSO, K25-Q223) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-2 amount of sample captured in plate. Background: Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Human Intercellular adhesion molecule 2 Protein information
  • Uniprot ID ICAM2_HUMAN
  • Uniprot AC P13598; Q14600;
  • UniGene Hs.431460;
  • GeneID 3384
  • KEGG hsa:3384;
  • Human Intercellular adhesion molecule 2 Protein SEQUENCE
  • SEQUENCE 275 AA; 30654 MW; C54FB34D92A6FC38 CRC64;

    MSSFGYRTLT VALFTLICCP GSDEKVFEVH VRPKKLAVEP KGSLEVNCST

    TCNQPEVGGL ETSLDKILLD EQAQWKHYLV SNISHDTVLQ CHFTCSGKQE

    SMNSNVSVYQ PPRQVILTLQ PTLVAVGKSF TIECRVPTVE PLDSLTLFLF

    RGNETLHYET FGKAAPAPQE ATATFNSTAD REDGHRNFSC LAVLDLMSRG

    GNIFHKHSAP KMLEIYEPVS DSQMVIIVTV VSVLLSLFVT SVLLCFIFGQ

    HLRQQRMGTY GVRAAWRRLP QAFRP





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