Intended UseHuman ICAM-1 ELISA Kit allows for the in vitro quantitative determination of ICAM-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalProduct Information: ICAM-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human ICAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.
Background: Biobool's human ICAM1 (Intracellular adhesion molecule 1 or CD54) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for ICAM-1 coated on a 96-well plate. Standards (NSO, Q28-E480) and test samples are added to the wells and ICAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human ICAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Application- Quantitative protein detection, establishing normal range